Journal: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Article Title: The Promises and Challenges of Tumor Mutation Burden as an Immunotherapy Biomarker: A Perspective from the International Association for the Study of Lung Cancer Pathology Committee

doi: 10.1016/j.jtho.2020.05.019

Figure Lengend Snippet: Examples of Targeted DNA-Based Assays That Generate a TMB Score

Article Snippet: 55 – 57 table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Assay Total size/Coding (Mb) Aberration in Algorithm a Germline Filtering Cancer Gene Bias Correction FFPE Error Correction Targeting Reference MSK-IMPACT 1.5/1.14 SNV (NS), indels Paired normal No Pool of normals Hybrid capture 15 FoundationOne CDx 2.2/0.8 SNV (NS, S), indels Database, SGZ Yes Bioinformatic Hybrid capture 55 Illumina TSO500 1.9/1.3 SNV (NS, S), indels Database, SGZ Yes UMI Hybrid capture 59 Thermo Fisher Oncomine 1.7/1.2 SNV (NS) Database No UDG; deamination metric Amplicon 56 Qiagen QIAseq TMB 1.3/1.3 SNV (NS), indels Database No UMI Amplicon Personal communication, Raed Samara, Qiagen NEO New Oncology, NEOplus ROU 2.5/1.2 SNV (NS), indels Database NA Bioinformatic Hybrid capture 76 Caris SureSelect XT 1.6/1.4 SNV (NS), indels Database No Bioinformatic Hybrid capture 57 Open in a separate window FFPE, formalin-fixed paraffin-embedded; indel, insertion-deletion; MSK-IMPACT, Memorial Sloan Kettering–Integrated Mutation Profiling of Actionable Cancer Targets; NA, not available; NS, nonsynonymous; S, synonymous; SGZ, somatic-germline zygosity algorithm; SNV, single nucleotide variants; TMB, tumor mutational burden; UDG, uracil-DNA glycosylase; UMI unique molecular identifier. a Aberrations included in the bioinformatic pipeline to estimate TMB.

Techniques: Amplification

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Representative figure showing the correspondence between the viral load during SARS-CoV-2 infection and the clinical course of the disease. The diagnostic windows of nucleic acid amplification tests (NAAT) and serology test are shown. Testing before and after the NAAT diagnostic window will show a false negative result . Nevertheless, testing before the serology diagnosing window will show in false negative results .

Article Snippet: KRISHGEN BioSystems , GENLISA™ Anti-SARS-Cov-2 (Covid-19) IgG/IgM Rapid Test , KBR011 , Qualitative determination of IgG/IgM antibodies to Covid-19 (SARS-CoV-2) , Immunochromatography with colloidal gold conjugate , Sensitivity ≥ 80% Specificity: ≥ 98% , CE-IVD.

Techniques: Infection, Diagnostic Assay, Amplification

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Specifications of the available polymerase chain reaction (PCR) commercial kits for laboratory diagnosis of COVID-19.

Article Snippet: KRISHGEN BioSystems , GENLISA™ Anti-SARS-Cov-2 (Covid-19) IgG/IgM Rapid Test , KBR011 , Qualitative determination of IgG/IgM antibodies to Covid-19 (SARS-CoV-2) , Immunochromatography with colloidal gold conjugate , Sensitivity ≥ 80% Specificity: ≥ 98% , CE-IVD.

Techniques: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Multiplex Assay, Quantitative RT-PCR, Fluorescence, In Silico, Acid Assay, Detection Assay, Diagnostic Assay, Amplification, Reverse Transcription Polymerase Chain Reaction

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Estimation of biomarker levels during the COVID-19/SARS-CoV-2 infection.

Article Snippet: KRISHGEN BioSystems , GENLISA™ Anti-SARS-Cov-2 (Covid-19) IgG/IgM Rapid Test , KBR011 , Qualitative determination of IgG/IgM antibodies to Covid-19 (SARS-CoV-2) , Immunochromatography with colloidal gold conjugate , Sensitivity ≥ 80% Specificity: ≥ 98% , CE-IVD.

Techniques: Biomarker Assay, Infection

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Specifications of the commercially available rapid serological tests for laboratory diagnosis of COVID-19.

Article Snippet: KRISHGEN BioSystems , GENLISA™ Anti-SARS-Cov-2 (Covid-19) IgG/IgM Rapid Test , KBR011 , Qualitative determination of IgG/IgM antibodies to Covid-19 (SARS-CoV-2) , Immunochromatography with colloidal gold conjugate , Sensitivity ≥ 80% Specificity: ≥ 98% , CE-IVD.

Techniques: Infection, Recombinant, Labeling, Immunochromatographic Assay, Diagnostic Assay, Binding Assay, Antigen Assay